Formulation and Antioxidant Test of Chromolaena odorata Leaf Extract in Gel with DPPH Method (1,1-Diphenyl-2-Picril Hydrazil)

Abstract

Chromolaena odorata leaf extract contains phenolic compounds that have activity as powerful antioxidants that can counteract free radicals. Free radicals are ions that are triggered by UV rays that can cause aging. The Antioxidant can stabilize free radicals so that overcome aging of the skin. The purpose of this study was to find out the most optimal formula of gel and to know its antioxidant activity. Chromolaena odorata leaf extract was obtained by maceration method by using ethanol 70%. This gel is formulated using CMC-Na as gelling agent and with dose variation of extract. The evaluation of gel were physical characteristic (spreadability and adhesivity) and tested for antioxidant activity using DPPH method. The results showed that percent inhibition of FI, FII, and FIII were 8.77±0.39, 9.33±0.09, and 56.6±0.13 respectively. The antioxidant activity of FIII exceeded 1.5 times the vitamin C (p<0.05) which is a powerful antioxidant. This is supported by testing the physical properties of the preparation according to the pH of the skin which is 5.93±0.17 does not irritate the skin. Gel preparations also have good homogeneity, dispersion, and adhesion. The formulation of Chromolaena odorata leaf extract in gel contains powerful antioxidants that have capability as antiaging.