UJI SITOTOKSIK FRAKSI DARI EKSTRAK METANOL RUMPUT GONG (Eriocaulon cinereum R.Br) pada SEL KANKER SERVIKS dan SEL NORMAL (VERO)

Abstract

Eriocaulon cinereum R. Br., known as rumput gong, has been used by the people in Bangka Belitung as traditional medicine to prevent uncontrolled cell growth. Preliminary study reported that the ethanol extract of E. cinereum was able to inhibit cell division of Hela cervical cancer with IC50 value of 427.79 μg/ml. This showed that this herb is potential to be developed as anticancer. A further study was to evaluate the cytotoxic activity of the fractions from the methanol extract of E. cinereum on Hela cell. The aim of this study was to examine the cytotoxic activity of fraction extract methanol of Rumput Gong (Eriocaulon cinereum R. Br.) against cervical cancer cells (HeLa) and normal cells (Vero). E. cinereum was extracted by ultrasound assisted maceration using n-hexane followed by ethyl acetate and then methanol. The extract was subjected by using separation tunnel method to obtain dichloromethane, chloroform and water fractions. The cytotoxic activity was evaluation by MTT assay method, the absorbance value was obtained using ELISA reader with the wavelength of 595 nm. Due to the amount of the chloroform fraction, no activities evaluation was carried out. The data were analyzed using PROBIT method from SPSS 16 for Windows®. The fraction of dichlorometane from methanol extract of E. cinereum showed cytotoxic activity with IC50 value of 235.652μg/mL. The water fraction has no cytotoxic activity due because its IC50 value was 2325.302μg/mL, selectivity index value of fraction dichlorometan was 2.09 and water fraction was 2.13. The dicholorometane fraction from methanol extract of E. cinereum has cytotoxic activity against Hela cell. Keywords