Pengembangan Metode Analisis Metil Paraben Dan Propil Paraben Pada Sediaan Cream Secara Simultan Dengan Metode Spektrofotometri UV/VIS

Abstract

The development methods of analysis using derivative spectrophotometric method, which is one of the spectrophotometric method which can be used for analysis directly on mixtures of several substances without making a separation, although with an adjacent wavelengths.. The aims of this study to determine the mixture of methyl paraben and propyl parabens in cosmetic creams were analyzed by spectrophotometric method UV / Vis derivatives, to determine a development method of analysis has a good value of validation, as well as to determine levels of methyl paraben and propyl paraben in the preparation creams accordance with BPOM. Spectrophotometry UV / Vis used as a method of analysis for preservatives because the method is simple, sensitive, and inexpensive operating costs. The analysis was conducted using content-Derivative Spectroscopy First order with a wavelength of 242.4 nm and 242.6 nm. The results of method validation show this method has a good validity for all parameters according to the AOAC validation. This method has a good linearity with r> 0.99. Precision with RSD values for methyl paraben at a wavelength of 242.4 nm and 242.6 nm respectively 4.04% and 3.78%, while for propyl paraben respectively 0.36% and 0.39 %. This method has a good accuracy with the average value percent recovery for methyl paraben at a wavelength of 242.4 nm and 242.6 nm respectively in the range of 93.58 to 118.86% and 82.75 to 112.93 %, while for propyl paraben is from 83.33 to 102.29% and 84.61 to 101.82%. Preservative levels of methyl paraben sample is 6,015 x10-4% w / w whereas propyl paraben 0.02% w / w. These levels were obtained did not exceed the maximum safe limit according to Food and Drug Administration (BPOM), there are no more than 0.4% w / w.