UJI ANTIKANKER EKSTRAK N-HEKSAN DAUN SORGUM (Sorghum bicolor L.) DENGAN METODE MTT ASSAY PADA SEL T47D

Abstract

Cancer is a group of cells that grows continuously in the tissues. One type of cancer that affects many women is breast cancer. Breast cancer is characterized by a lump in the breast that experiences uncontrolled cell development. Sorghum plants (Sorghum bicolor L.) are identified as having steroid and alkaloid contents which are thought to inhibit the development of cancer cells. The purpose of this study was to determine the content of chemical compounds contained in sorghum (Sorghum bicolor L.) leaf extracts and determine the inhibitory activity of sorghum n-hexane extract (Sorghum bicolor L.) against breast cancer by MTT-Assay method. The research was begun by taking sorghum leaves, washing, sorting, drying with cabinet dryer and extraction. Extraction was carried out applying n-hexane solvent using maceration method that was assisted with sonicator which was then followed by phytochemical screening tests to determine the compounds contained in sorghum leaves. The activity of n-hexane extract on breast cancer cells was carried out by MTT-Assay method and data analysis using statistical tests. Phytochemical screening test results of sorghum leaf extract showed that n-hexane extract contained alkaloids and steroids. IC50 ± SD values obtained from the anticancer test on T47D cells were 1374.437 μg / mL ± 121.299 μg / mL while in Vero cells 1717.94 ± 300.942 μg / mL were obtained. The IC50 ± SD value indicates that the extract was categorized as not having breast anticancer activity.