Chemical compatibility of midazolam and morphine in 5% glucose solution

Abstract

To date, data on compatibility of midazolam and morphine in 5% glucose under ambient temperature and light are lacking. This study assayed compatibility of midazolam and morphine after reconstitution under conditions commonly practiced in the hospital. Solution of midazolam and morphine was prepared by diluting 5% glucose to a 50 mL syringe injection to a final concentrations of 0,58 mg/mL for Midazolam and 96 μg/mL for morphine were achieved. The triplicate solutions were stored at ambient temperature under either mixed daylight. The aliquot sample was taken from syringe at times 0, 8, 24, 72, 120, and 168 hours. The aliquot part solutions were examined for visual inspection, pH and concentration changes assayed by highperformance liquid chromatography. The sample of midazolam and morphine were clear, no turbid, no gas, and no colour changes during 168 hours. The concentrations of midazolam, and morphine at 168 hours were retained ±90% compared to freshly prepared solution. This result concluded that solutions of midazolam and morphine in 5% glucose could be stored in syringes injection at ambient temperature up to 168 hours.