Uji Aktivitas Antibakteri dan Antibiofilm Ekstrak Etil Asetat Batang Jatropha Multifida Linn terhadap Staphylococcus Aureus ATCC 25923 dan MRSA

Abstract

Jatropha multifida Linn or often called the iodine tree is a shrub that is commonly found in Indonesia. Chemical compounds of Jatropha multifida Linn have antimicrobial ability against gram-positive bacteria such as Methicilin-resistant S. auresus (MRSA), S. aureus, and S. Epedermis. In addition to having antimicrobial ability, Jatropha multifida L multifact also has antifungal activity in C.alba (ATCC 1023), and antimalarial to Plasmodium falciparum. The purpose of this study was to determine the antibacterial and antibiofilm activity of Jatropha multifida Linn stem extract. The method used in this plant extraction process is by soxhletasi method. To know the antibacterial activity of the extract was done by microdilution method followed by the more specific activity test that is the test of microbial activity of antibiofilm. Absorbance of bacterial biofilm colonies was measured by ELISA reader. The resulting microdilution resulted in KHM of 1250 μg/mL and KBM of 2500 μg/mL for Staphylococcus aureus ATCC 25923 and MRSA. The result of antibiofilm test showed that biofilm inhibition in Staphylococcus aureus ATCC 25923 bacteria and MRSA with IC50 were 2225 μg/mL and 2643 μg/mL respectively. And from this research it is found that biofilm can be more inhibited by the ethyl acetate extract of Jatropha multifida L stem in ATCC 25923 Staphylococcus aureus bacteria than in MRSA-treated bacteria. This can be seen from the value of IC50 obtained, because from previous research IC50 is good in plants in inhibiting biofilm is at IC50 <3200 μg/mL.